a possibility, if you must try, would be to look at intein-based protein
ligation. Hideo Iwai has a series of papers on this over the last 15+ years.
Adrian
On 26 Jul 2023, at 11:14, Armando Albert
mailto:xalb...@iqfr.csic.es>> wrote:
Dear all,
We are trying to characterize a protein consistin
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] On the production of a two domain protein
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Dear all,
We are trying to characterize a protein consisting of two domains. We have
Hi Armando
At the risk of appearing flippant, why not submit the sequence of the
full-length protein to something like Alphafold (readily available to all via
ColabFold (see
https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb)?
I suspect that each of the t
Dear all,
We are trying to characterize a protein consisting of two domains. We have
successfully produced, purified and crystallized both domains independently.
However, we are unable to overexposes a soluble form of the full-length
protein. Can anyone provide a strategy to merge back the ind
