a possibility, if you must try, would be to look at intein-based protein ligation. Hideo Iwai has a series of papers on this over the last 15+ years.
Adrian On 26 Jul 2023, at 11:14, Armando Albert <xalb...@iqfr.csic.es<mailto:xalb...@iqfr.csic.es>> wrote: Dear all, We are trying to characterize a protein consisting of two domains. We have successfully produced, purified and crystallized both domains independently. However, we are unable to overexposes a soluble form of the full-length protein. Can anyone provide a strategy to merge back the independent domains into a single protein chain or to modify the construct to succeed in the overproduction of the full-length protein? Armando ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ Adrian Goldman, Ph. D., FRSB MIBS, Biological and Environmental Sciences, P. O. Box 56, Viikinkaari 9C FIN-00014 University of Helsinki Finland Tel: +358 29 41589087 email: adrian.gold...@helsinki.fi<mailto:adrian.gold...@helsinki.fi> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
