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Subject: [ccp4bb] Off-topic (SPR)
Dear pals,
Sorry for my off-topic question but its been a while I am challenging to get a
15 kDa protein coated on CM5 chip. Calculated PI of protein is 5.9 & I tried
Acetate buffer pH5.2 and 3.8 for coating under normal procedure
Dear pals,
Sorry for my off-topic question but its been a while I am challenging to get a
15 kDa protein coated on CM5 chip. Calculated PI of protein is 5.9 & I tried
Acetate buffer pH5.2 and 3.8 for coating under normal procedure of Biacore.
After immobilization I saw this message: response lev