Dear pals, Sorry for my off-topic question but its been a while I am challenging to get a 15 kDa protein coated on CM5 chip. Calculated PI of protein is 5.9 & I tried Acetate buffer pH5.2 and 3.8 for coating under normal procedure of Biacore. After immobilization I saw this message: response level decreased! This happened for me several times. Does anyone have any idea what's going on?
Cheers, Jahan
