Hi
An easy fix is to PCR amplify your low copy number plasmid (modern
polymerases have no issues amplifying up to 20 kB if you have a largeish
vector) and then sequence the product. Alternatively you can amplify just a
portion of interest, of course (you said sequence the plasmid so I went
with yo
The pACYC vectors (probably your p15ori plasmid) is said to have a copy
number of ~10 copies per cell while your other plasmid is likely in the
hundreds - see:
https://blog.addgene.org/plasmid-101-origin-of-replication
The protein expression level from the lower copy number plasmid is
usually
Hi All,
I have two constructs having different ori, p15ori and M13 ori, different
promoters araBAD promoter and LacI, and different antibiotic resistance
chloramphenicol and Ampicillin respectively.
I am managed to get the transformants and getting the expected result after
blunt digestion with th
