Hello Jacob,
The problem for native gel is that it is much more sensitive to a
single charge difference than size differences. Also, the gel pattern
may change greatly if you use a different buffer system. I had a case
2 years ago that my protein ran at 5 positions on a Laemmli(pH 8.8)
na
in board [mailto:[EMAIL PROTECTED] On Behalf Of Jacob
Keller
Sent: Wednesday, November 19, 2008 3:57 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Native Gel Charge States Vs. Conformations Vs. Oligomeric
States
Dear Crystallographers,
I am having trouble interpreting the attached native gel (which is
Somebody asked about cysteines:
only one cysteine (therefore only dimers possible), but the native gels are
exactly the same with lots of DTT anyway.
JPK
***
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laborato
Jacob - To me it looks like your data are inconsistent. I would first
clear up the apparent discrepancy between your AUC and SEC data.
Regarding the native gel, you seem to have a mixture of fairly stable
species that don't interconvert much. If mass spec gives you only one
species, I would
Dear Crystallographers,
I am having trouble interpreting the attached native gel (which is very
repeatable, under various conditions). Is it tenable that various charge
states or conformations could account for this behavior, or must it be
various oligomeric states? AUC results seem to show on
