Jacob - To me it looks like your data are inconsistent. I would first
clear up the apparent discrepancy between your AUC and SEC data.
Regarding the native gel, you seem to have a mixture of fairly stable
species that don't interconvert much. If mass spec gives you only one
species, I would rule out deamidation products (i.e., differently
charged species), but oligomeric species is still a possibility. That
would clash with your AUC data, so perhaps something is wrong with the
underlying experiments. Hope that helps. Best - MM
--------------------------------------------------------------------------------
Mischa Machius, PhD
Associate Professor
Department of Biochemistry
UT Southwestern Medical Center at Dallas
5323 Harry Hines Blvd.; ND10.214A
Dallas, TX 75390-8816; U.S.A.
Tel: +1 214 645 6381
Fax: +1 214 645 6353
On Nov 19, 2008, at 2:56 PM, Jacob Keller wrote:
Dear Crystallographers,
I am having trouble interpreting the attached native gel (which is
very repeatable, under various conditions). Is it tenable that
various charge states or conformations could account for this
behavior, or must it be various oligomeric states? AUC results seem
to show only monomers, whereas SEC shows a series of peaks. Mass-
spec shows no post-translational mods. Anyone have any similar
experiences, or references?
Thanks,
Jacob
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
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<native_gel.jpg>
