If it's the M1 antibody, you can elute with EDTA + Flag peptide.
You could also try a little higher pH like 3.5.
Ho Leung Ng
University of Hawaii at Manoa
Assistant Professor, Department of Chemistry
holeung...@hawaii.edu
On Thu, Nov 17, 2011 at 2:00 PM, CCP4BB automatic digest system
wr
Hi all,
Sorry for the off-topic question, but I wanted to find out anyone's experience
with using magnetic anti-flag beads (Sigma). I have been having a problem with
the anti-flag antibody leaching from the beads during low pH elution ( which
shouldn't be happening I am thinking) and was wonder
