If it's the M1 antibody, you can elute with EDTA + Flag peptide. You could also try a little higher pH like 3.5.
Ho Leung Ng University of Hawaii at Manoa Assistant Professor, Department of Chemistry holeung...@hawaii.edu On Thu, Nov 17, 2011 at 2:00 PM, CCP4BB automatic digest system <lists...@jiscmail.ac.uk> wrote: > From: "Harman, Christine" <christine.har...@fda.hhs.gov> > Subject: Magnetic anti-flag beads > > Hi all, > Sorry for the off-topic question, but I wanted to find out anyone's > experience with using magnetic anti-flag beads (Sigma). I have been having a > problem with the anti-flag antibody leaching from the beads during low pH > elution ( which shouldn't be happening I am thinking) and was wondering if > anyone has had a similar experience. I have talked to Sigma about this and > they sent me another lot of beads which also appears to be leaching antibody > during incubation with 0.1M glycine pH 2.7 (normal elution described in > protocol except at pH 3.0). I am not using the beads for purification, but > will be using them for protein interactions studies so sorry about the > off-topic question. Given that you all are a very smart community I thought > I would throw this one out to you. > > Thanks for any brain waves on this, > > Christine
