Re: [ccp4bb] MS sequencing of Fab

2011-05-23 Thread Sean Seaver
>do not diffract to sufficiently high resolution so as to allow us to read off >the sequence from the map). >Since we have access to a lot of pure protein, I wonder if some clever mass >spec jock would be able to assemble >enough overlapping sequenced fragments so >as to give complete coverage

[ccp4bb] MS sequencing of Fab

2011-05-23 Thread Patrick Loll
We've crystallized a complex of an Fab bound to a protein. We have the hybridomas from which the Fab was prepared, but no protein sequence for the antibody. We're trying to plot the easiest course to get the sequence (since the crystals, alas, do not diffract to sufficiently high resolution so a