>do not diffract to sufficiently high resolution so as to allow us to read off >the sequence from the map).
>Since we have access to a lot of pure protein, I wonder if some clever mass >spec jock would be able to assemble >enough overlapping sequenced fragments so >as to give complete coverage of the protein. Has anyone done this/had >this >done for them? Hi Pat, Here is an example... Hemoglobin redux: combining neutron and X-ray diffraction with mass spectrometry to analyse the quaternary state of oxidized hemoglobins http://journals.iucr.org/d/issues/2010/11/00/dz5208/dz5208bdy.html (see Results section 3.1) of combining X-ray crystallography and mass spect. to attain a full sequence. Since you mentioned attaining low resolution crystallographic data it may not be possible to distinguish between isoleucine and leucine. However, you should be able to determine most of the sequence via mass spect. Take Care, Sean P212121 http://store.p212121.com/
