867-872
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From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Patrick Loll
Sent: Wednesday, September 05, 2007 12:42 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] MS for verification of protein constructs
I wonder if anyone would care to share experiences/ideas/biases
Hiya
For verification purposes we almost always N-terminal sequence (we are
fortunate in that we have such a facility on site so that the samples can be
turned round fast) - old technology but good and solid! We then usually
combine these data with a Mass Spectra (MALDI-ToF-Tof - on site - is
... all these are correct indeed - but ESI-TOF is also a nice
solution, especially coupled to an LC system.
My understanding was that MALDI-TOF is better for smaller fragments,
accuracy can be about 10 Dalton ...
for more info there is a useful short review of the use of ms
techniques for s
If you have a very pure protein sample, you'll want to use an ESI-ion trap
for analyzing proteins of that size. It should be possible to get an exact
mass (i.e. within a single Da). It's possible, but very rare, to get exact
masses of proteins up to 100 kDa using ESI-ion trap instruments.
If you
I second Dr. Loll's question, and would like to be CC'd in whatever MS tips,
including
service-providers, are sent. I have been having a bit of a debacle with a
certain MS service provider.
Jacob Keller
==Original message text===
On Wed, 05 Sep 2007 11:41:52 am CDT Patri
I wonder if anyone would care to share experiences/ideas/biases that
relate to the use of mass spectrometry to verify the identity of
protein constructs used for crystallization. Our experience with
different MS facilities has been checquered.
Specifically:
What's the current thinking on
