I wonder if anyone would care to share experiences/ideas/biases that
relate to the use of mass spectrometry to verify the identity of
protein constructs used for crystallization. Our experience with
different MS facilities has been checquered.
Specifically:
What's the current thinking on the best approach to get masses for
intact proteins of moderate size (say, 40 kD)? ESI-TOF?
What kind of resolution should one hope to obtain in such cases
(10E-04?)
Any suggestions as to good facilities offering fee for service MS
characterization are welcome (but should be shared off line, I think;
continental US only).
Thanks,
Pat
------------------------------------------------------------------------
---------------
Patrick J. Loll, Ph. D. (215) 762-7706
Associate Professor FAX: (215) 762-4452
Department of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA
[EMAIL PROTECTED]
