: Meetmr Ss
Cc: CCP4BB@jiscmail.ac.uk
Sent: Tuesday, December 30, 2008 2:04:24 PM
Subject: Re: [ccp4bb] MR- Problem-76 % seqeunce identity-no solution
Meetmr Ss wrote:
> Dear all,
>
> I struck with MR problem. MY target has 76% sequence identity with the model.
> I tried Phaser, AMoR
Meetmr Ss wrote:
Dear all,
I struck with MR problem. MY target has 76% sequence identity with the model. I tried Phaser, AMoRe and Molrep. None of them gave me satisfactory solution. If you have any suggestions and or New programs I would like to try.
Thanks
somu
Is your space g
I had the same problem with an even higher %identity once (>80%), eventually
resorted to SIRAS phasing with a mercury compound, and I found a case of
domain swapping creating a novel dimer interface.
Flip
Hi Somu,
Here are a few questions which might help:
Are you sure about about your space group? Is another lattice/symmetry
possible? Is twinning possible?
Have you tried all possible space groups in MR?
Have you tried different resolution ranges (e.g. all data, cut low
resolution at 15 A, cut hi
I struck with MR problem. MY target has 76% sequence identity with the
model. I tried Phaser, AMoRe and Molrep. None of them gave me satisfactory
solution. If you have any suggestions and or New programs I would like to
try.
that is odd. at 76% SI mol. repl. should normally be a breeze. possib
Dear all,
I struck with MR problem. MY target has 76% sequence identity with the model. I
tried Phaser, AMoRe and Molrep. None of them gave me satisfactory solution. If
you have any suggestions and or New programs I would like to try.
Thanks
somu
