I guess it depends on your criteria for success. We made a successful MR using
data to 8Å with a search-model with 20% identity covering 90% of the target. The
resultant phases gave a map where a few new 'blobs' could be observed. Not very
useful, but the MR-phases could be used to solve the HA
Hi,
I cannot really answer what the lowest resolution for MR is but I have
been successful with 4 A data for a protein-DNA complex and so I
encourage you to try your 3.6 A data set. Of course, it also depends on
the quality of your data, in particular how well/how many low resolution
reflections we
If you look at the molecular replacement search parameters, you will
find that the rotational and translational searches can be done at 4
Angstrom or lower values assigned to the 'high resolution' values.
So the real worry in your case, in all likelihood, is not whether MR
will work for 3.
What is the lowest resolution one can try to do molecular replacement with?
I have a 3.6 angstroms resolution data for a protein-DNA complex and
wondering whether I can try MR to see the density for DNA.
