If you look at the molecular replacement search parameters, you will
find that the rotational and translational searches can be done at 4
Angstrom or lower values assigned to the 'high resolution' values.
So the real worry in your case, in all likelihood, is not whether MR
will work for 3.6 Ang resolution data. The greater worry is as to how
much model bias gets introduced at that resolution and as to how well
one can refine the model only based on the phases from molecular
replacement.
I can't tell from your description if you have DNA in your search
model or not. But If you get a molecular replacement solution and if
at the least, some bits of your DNA molecules are ordered, you will
see big bloopers of density that will correspond to the phosphate
molecules. The density for sugars and base-pairs may or may not be
well defined in the initial stages.
Raji
On Mar 30, 2009, at 12:25 PM, Muthiah wrote:
What is the lowest resolution one can try to do molecular
replacement with? I have a 3.6 angstroms resolution data for a
protein-DNA complex and wondering whether I can try MR to see the
density for DNA.
