Re: [ccp4bb] Large scale insect cell expression

2018-12-11 Thread radu
Hi Joseph, Apologies for not answering your question really :-) But, unless you plan to focus on very peculiar insect cell proteins, I would suggest considering HEK cells. See PMID: 30455477 for one of several possible systems. Cheaper, easier, wave bag-friendly... Overall better than insect, espe

[ccp4bb] Large scale insect cell expression

2018-12-11 Thread Joseph Brock
Dear ccp4 community, I'll soon start my own lab and together with other colleagues, would like to establish a facility to do large scale insect cell culture for crystallography. Has anyone had any experience with the wave bag systems for this purpose (for instance from GE)? I have heard that c