Dear Rohit,
If your protein has a spectroscopic signature corresponding to the different
redox states, you could try using single crystal spectroscopy (e.g. UV-visible)
to validate the redox state before and after data collection.
Best wishes,
Mike
On 5 Jan 2018, at 10:45, rohit kumar
mai
/www.ncbi.nlm.nih.gov/pmc/articles/PMC3617268/
>
> Bests,
> Pierre Legrand
> PROXIMA-1 synchrotron-SOLEIL
>
> --
> *De :* CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de rohit
> kumar [rohit...@gmail.com]
> *Envoyé :* vendredi 5 janvier 2018
5
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Iron Oxidising state
Dear All,
I am solving a structure (resolution 2 A) of an enzyme having Iron as a metal
cofactor.
How could I know oxidising state of the bound Iron (Fe+2 or Fe+3)?
Thank you in advance
--
WITH REGARDS
Dr. Rohit Kumar Singh
Dear All,
I am solving a structure (resolution 2 A) of an enzyme having Iron as
a metal cofactor.
How could I know oxidising state of the bound Iron (Fe+2 or Fe+3)?
Thank you in advance
--
WITH REGARDS
Dr. Rohit Kumar Singh
