Hi, Due to the absorbed X-ray dose and photoreduction process during datacollection, it is most probably Fe+2. See for example: J Biol Chem<https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617268/#>. 2013 Apr 5; 288(14): 9648–9661. doi: 10.1074/jbc.M112.438796<https://dx.doi.org/10.1074%2Fjbc.M112.438796> https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3617268/
Bests, Pierre Legrand PROXIMA-1 synchrotron-SOLEIL ________________________________ De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de rohit kumar [rohit...@gmail.com] Envoyé : vendredi 5 janvier 2018 11:45 À : CCP4BB@JISCMAIL.AC.UK Objet : [ccp4bb] Iron Oxidising state Dear All, I am solving a structure (resolution 2 A) of an enzyme having Iron as a metal cofactor. How could I know oxidising state of the bound Iron (Fe+2 or Fe+3)? Thank you in advance -- WITH REGARDS Dr. Rohit Kumar Singh
