bject:
[ccp4bb] How to separate BSA present in Thrombin
Hi all,
My protein size is nearly the same size of BSA and it has His tag. If I
have to cleave the tag I have to use Thrombin which is having huge amount
of BSA in it. Now how will I separate this BSA from my protein.
Thanks and Regards,
Manj
sible.
HTH.
Nicolas
De : CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] de la part de Manjula Ramu
[manjula@gmail.com]
Envoyé : mercredi 8 octobre 2014 15:27
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] How to separate BSA present in Thrombin
Hi all,
My protein si
Dear Manjula,
Unless Thrombin requires BSA for functioning (I never used Thrombin) you
could run a size exclusion column with the Thrombin solution prior to
using it. Or you could by it from a different vendor.
Regards,
Tim
On 10/08/2014 03:27 PM, Manjula Ramu wrote:
> Hi all,
>
> My protein si
Hi all,
My protein size is nearly the same size of BSA and it has His tag. If I
have to cleave the tag I have to use Thrombin which is having huge amount
of BSA in it. Now how will I separate this BSA from my protein.
Thanks and Regards,
Manjula R
Research Scholar
Department of Biophysics
Nationa
