Hi all, My protein size is nearly the same size of BSA and it has His tag. If I have to cleave the tag I have to use Thrombin which is having huge amount of BSA in it. Now how will I separate this BSA from my protein.
Thanks and Regards, Manjula R Research Scholar Department of Biophysics National Institute of Mental Health and Neurosciences Bengaluru-29, Karnataka INDIA E-mail: manjula....@gmail.com Mobile no:+91-9538553356 http://www.nimhans.kar.nic.in/ <https://www.nimhans.kar.nic.in/>