Dear All,
Thank you very much for all your suggestions. They are very helpful in my
further refinements. I am adding some more information about the problem:
1) The Z-score is 11.2 and LLG is 125.
2) The model has 390 aa while my structure has about 440 aa.
Please let me know of any further su
I've found CNS's simulated annealing composite omit maps to be
very useful in situations like this to avoid phase bias. RESOLVE's
prime and switch offers similar functionality, but I've had less
experience with it.
ho
UC Berkeley
Dear Sun,
if I remember correctly phaser already carries out a rigid body refinement
of its solution.
To avoid and remove as much model bias as possible, I suggest you look at
the solution from phaser and build _as_ _much_ _as_ _possible_ BEFORE you
do any further refinement, i.e., try to ma
Hi Sun,
For 2.5A data you can try phenix or arp/warp to autobuild the the model
using the phase form MR ,then you can get the most of model you want if
you are lucky enough or you need more manual bulding or another cycle MR
again .
Good luck!
liu
Sun Tang wrote:
Dear All,
Recently I solved
Dear All,
Recently I solved a structure at 2.5 A with PHASER, with two molecules in
assymetric unit. There are no short contacts in the solution. However, the
Rfree stays at about 50% after the refinement of rigid boby and restrained
refinement in CNS. The sequences homology is about 30%.
Wha
