Hi Sun,
For 2.5A data you can try phenix or arp/warp to autobuild the the model
using the phase form MR ,then you can get the most of model you want if
you are lucky enough or you need more manual bulding or another cycle MR
again .
Good luck!
liu
Sun Tang wrote:
Dear All,
Recently I solved a structure at 2.5 A with PHASER, with two molecules
in assymetric unit. There are no short contacts in the solution.
However, the Rfree stays at about 50% after the refinement of rigid
boby and restrained refinement in CNS. The sequences homology is about
30%.
What is the usual way to deal with this kind of problems? Should I
just refine the backbone of the structure and then build the side chains?
Your suggestions are greatly appreciated!!!
Best wishes,
Sun
