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> *From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of *Shu
> XU
> *Sent:* 06 September 2009 17:21
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] How to get rid of the skin in the screening drop
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> Lower the concentratio
Lower the concentration did help a little bit. I didn't the 6 mg/ml setups,
but still got some skins.
You are right the skins are more likely to find in the condition of
PEGs...but also find in other conditions.
Lower the conc. of PEGs is a good advice, and I'll try it. Thanks.
Shu
On Sun, Sep 6,
This happened to me previously, and the only thing that worked for me was to
lower the protein concentration. I was setting my protein up at 10 mg/mL,
only to find skins on nearly all the drops. I would damage the crystals
trying to get them out of the drops... I lowered my protein concentration
Hi, all.
I'm doing the crystallization screening for my protein.
In over 1/2 of my hanging drops, there were skins formed on the surface of the
drop.
So I switched to use microbatch, and again almost 4/5 drops under the oil were
skins at the interface.
I tried to add additives, like detergents, o
