I would certainly try cocrystallizing with adp, amppnp, or ATPgS
Many helicases have been crystallized in this way. Papers by Sawaya et al and
Singleton et al come readily to mind.
Best of luck
Savvas
On 09 Mar 2011, at 15:52, Ed Pozharski wrote:
> On Wed, 2011-03-09 at 10:52 +, Art wro
On Wed, 2011-03-09 at 10:52 +, Art wrote:
> No protein crystal was found after some typical conditions (kit I, kit
> II, and Index) were repeatly screened for more than three times.
See Einstein's definition of insanity :)
> Protein concentration gradient was considered, but it can not work.
Dear Art,
It seems twisted, indeed.
First of all you need to verify that your protein is at all in a good
state (you seem to imply that it is not with your remark on protein
concentration). Basic biophyscial characterisation for stability and
structural homogeniety is required to reach proper buf
Dear all!
We've been trying to crystallize a helicase. No protein crystal was found after
some typical conditions (kit I, kit II, and Index) were repeatly screened for
more than three times. 50 mM MES (pH 6.0), 5% glycerol, and 1mM DTT is used as
the final storage buffer. The most significant pr
