Dear Art, It seems twisted, indeed.
First of all you need to verify that your protein is at all in a good state (you seem to imply that it is not with your remark on protein concentration). Basic biophyscial characterisation for stability and structural homogeniety is required to reach proper buffer conditions for your protein, and you may consider a complex with relevant ligands Poul > Dear all! > We've been trying to crystallize a helicase. No protein crystal was found > after some typical conditions (kit I, kit II, and Index) were repeatly > screened for more than three times. 50 mM MES (pH 6.0), 5% glycerol, and > 1mM DTT is used as the final storage buffer. The most significant problem > of this protein is its precipitation in most of the screening conditions. > Protein concentration gradient was considered, but it can not work. > I was wondering if there is any way to work aroud this problem. Look > forward to your suggestions! > Thanks! > Art >
