Another thing you should bookmark and check out is the Molprobity page (google
for it), upload your pdb file and let it evaluate what you have done. The chart
it generates will guide you through your structure and you will be able to do a
good job.
Jürgen
..
Jürgen Bosch
Jo
Dear Xun,
> > I have a 3.2A dataset for a protein-DNA complex. The protein is
> > a homodimer, and the DNA is almost palindromic (except one base pair
> > in the middle and two or three base pairs at both two ends). It is my
> > first time solving structures, and unfortunately the resolutio
Thank you!! It's really encouraging & refreshing to begin a day with so many
advices! ccp4bb is always helpful~
Thanks to one of my committee member who suggested me to get out a paper
from the structure, and thanks to all the people at the mid-Atlantic
crystallography meeting who told me my struct
> I have a 3.2A dataset for a protein-DNA complex. The protein is
> a homodimer, and the DNA is almost palindromic (except one base pair
> in the middle and two or three base pairs at both two ends). It is my
> first time solving structures, and unfortunately the resolution is
> low. No body
Hi Xun,
I'd find out what the NCS averaging correlation is between the molecules in the
asymmetric unit. If your correlation is high then tight NCS should help in
refinement. You could also take a look at the NCS averaged map in COOT. You can
also have ideal helices build using COOT or PHENIX (
Hi Xun,
I have a 3.2A dataset for a protein-DNA complex. The protein is a
> homodimer, and the DNA is almost palindromic (except one base pair in the
> middle and two or three base pairs at both two ends). It is my first time
> solving structures, and unfortunately the resolution is low. No
On Thu, Jun 16, 2011 at 2:11 PM, Xun Lu wrote:
> I have a 3.2A dataset for a protein-DNA complex. The protein is a
> homodimer, and the DNA is almost palindromic (except one base pair in the
> middle and two or three base pairs at both two ends). It is my first time
> solving structures, an
Hi,
I have a 3.2A dataset for a protein-DNA complex. The protein is a
homodimer, and the DNA is almost palindromic (except one base pair in the
middle and two or three base pairs at both two ends). It is my first time
solving structures, and unfortunately the resolution is low. No body in ou
