Hi Xun, I have a 3.2A dataset for a protein-DNA complex. The protein is a > homodimer, and the DNA is almost palindromic (except one base pair in the > middle and two or three base pairs at both two ends). It is my first time > solving structures, and unfortunately the resolution is low. No body in our > lab has used ccp4 or phenix, so I am really frustrated as a second year > student.
just in case you want to get some overview/background, see http://www.phenix-online.org/presentations/latest/pavel_refinement_general.pdf and low-resolution specific, see pages 49-59. Pavel.