Hi Arpit,
You can use PEI (polyethyleneimine). Adding PEI at low conc. (e.g. 0.25%)
after cell lysis should precipitate most of the DNA. Note, it is best to do
the addition step-wise at 4 deg with gentle steering over a period of time
10-15 min or so.
Ibrahim
On 5/12/10 10:20 AM, "Tim Gr
Hello Arpit,
don't you use DNAse during lysation of the cells? Or do you mean that you want
to get rid of unspecific DNA and want to add some specific DNA after
purification? In that case I'd understand you don't want to use DNAse.
Tim
On Wed, May 12, 2010 at 06:23:56PM +0530, Arpit Mishra wrote:
Dear Arpita,
For Getting rid of DNA you can incubate the lysate with DNase and incubate
it before lysing the cells with sonication or french press (or anyother
method of your choice)
cheers
Rashmi
On Wed, May 12, 2010 at 8:53 PM, Arpit Mishra wrote:
>
>
> -- Forwarded message
-- Forwarded message --
From: Arpit Mishra
Date: Wed, May 12, 2010 at 6:20 PM
Subject: regarding purification of DNA binding protein
To: ccp...@jiscmail.ac
hi all
i am trying to purify DNA binding protein, but i couldnt get rid of the DNA
after performing hi-trap heparin column,
