Hi Arpit, You can use PEI (polyethyleneimine). Adding PEI at low conc. (e.g. 0.25%) after cell lysis should precipitate most of the DNA. Note, it is best to do the addition step-wise at 4 deg with gentle steering over a period of time 10-15 min or so.
Ibrahim On 5/12/10 10:20 AM, "Tim Gruene" <t...@shelx.uni-ac.gwdg.de> wrote: > Hello Arpit, > > don't you use DNAse during lysation of the cells? Or do you mean that you want > to get rid of unspecific DNA and want to add some specific DNA after > purification? In that case I'd understand you don't want to use DNAse. > > Tim > On Wed, May 12, 2010 at 06:23:56PM +0530, Arpit Mishra wrote: >> ---------- Forwarded message ---------- >> From: Arpit Mishra <ar...@igib.in> >> Date: Wed, May 12, 2010 at 6:20 PM >> Subject: regarding purification of DNA binding protein >> To: ccp...@jiscmail.ac >> >> >> hi all >> i am trying to purify DNA binding protein, but i couldnt get rid of the DNA >> after performing hi-trap heparin column, i have also tried high conc salt >> buffer (upto 1 M NaCl) and also tried o.2 M lithium sulphate, please suggest >> me some way to get rid of undesired DNA.. >> >> thanks >> >> Regards >> >> Arpit
