hanks everyone for keeping me frugal.
>
> Cheers,
> Christine
> --
> *From:* Chun Luo [mailto:c...@accelagen.com]
> *Sent:* Tuesday, April 19, 2011 3:18 PM
> *To:* Harman, Christine; CCP4BB@JISCMAIL.AC.UK
> *Subject:* RE: [ccp4bb] Flag tag vs. h
probably go with
His. Thanks everyone for keeping me frugal.
Cheers,
Christine
From: Chun Luo [mailto:c...@accelagen.com]
Sent: Tuesday, April 19, 2011 3:18 PM
To: Harman, Christine; CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] Flag tag vs. his-tag
His-tag is close to
Behalf Of
Harman, Christine
Sent: Tuesday, April 19, 2011 11:11 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Flag tag vs. his-tag
Hi all,
I am trying to decide between using a N-term his-tag or an N-term flag tag
to be expressed on a protein that I will eventually want to try and
crystallize
Hi all,
I am trying to decide between using a N-term his-tag or an N-term flag tag to
be expressed on a protein that I will eventually want to try and crystallize.
I usually don't cleave my tags unless absolutely necessary and I want to avoid
double tagging. I am leaning towards the flag since
