Re: [ccp4bb] E.coli strain, knock-out for SDH/FR

2017-04-04 Thread Edward A. Berry
People who would know (and know whether such a strain would even be viable) are Gary Cecchini at UC San Francisco/VA and Bob Gennis at Univ of Illinois at Urbana. In case you are not already talking with them. It has been reported that an assembly factor (SDH5 or SDHE or SDHAF2) is required for f

[ccp4bb] E.coli strain, knock-out for SDH/FR

2017-04-04 Thread Fulvio Saccoccia, Sapienza
Dear ccp4ers, I was wondering if someone does know (and hence could let me know in turn) an E.coli strain which lacks both of the sdh (succinate dehydrogenase) and frd (fumarate reductase) operon, so that bacteria do no longer retain any activity from complex II or similar. I know the que