Dear Wataru
check out the following entry on the CCP4-wiki on 'sticky crystals' which we
compiled based on feeback from many crystallographers:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Sticky_crystals
Best regards
Savvas
Savvas Savvides
Unit for Structural Biology @ L-P
Hi Wataru,
Have you tried Mitegen's polymer MicroTools? These tools have tips made
from soft, flexible microfabricated polymer films and are specifically
designed for this type of application.
There is more information about them here (
http://mitegen.com/products/microtools/microtools.shtml
Hi Wataru,
I second Bill's suggestion. Additionally, you might want to migrate to a
sitting drop setup and use the vacuum grease with that. It worked for me.
Happy Fishing,
Chris
--
Christopher L. Colbert, Ph.D.
Assistant Professor
Department of Chemistry and Biochemistry
North Dakota State U
Cool the coverslip on the opposite side of the crystal with a chip of dry
ice. Do not freeze the drop. I learned this from Gary Gilliland. Also I
wonder if you can simply move the whole tray into a cooler temperature?
You can imagine that the thermal expansion coefficient of the glass
cover
Have you tried seeding ?
And another suggestion, if the small ones already diffract to 3.5 Å on a home
source, why don't you try to go to a synchrotron ?
Big is also not always better, they might freeze worse than your small ones or
might have growth artifacts etc.
If you take several huge cryst
] Detaching crystals from glass cover slides
Hi all,
I have crystals growing by the hanging-drop method, using 24-well VDX plates
and Hampton Research siliconized glass cover slides. Most crystals are
attached to the cover slide, and I am having difficulties detaching the
crystals (using a cryoloop
Hi Wataru:
I hope all is well. For the ones you already have grown, try very gently
prying them off with a wedge-shaped needle.
If you can grow more, try using a very thin smooth layer of vacuum grease, and
apply the drop to that. I managed to get RNA crystals to grow that way that
otherwise
Hi all,
I have crystals growing by the hanging-drop method, using 24-well VDX plates
and Hampton Research siliconized glass cover slides. Most crystals are attached
to the cover slide, and I am having difficulties detaching the crystals (using
a cryoloop) without breaking them. There are few sm
