Dear Wataru-san: I understand the problem and sometimes I also faced the same difficulties. One likely suggestion to pick up the crystal is here: 1. Hold the cover-glass in hand (the drop should be facing bottom side as in the hanging drop setup) 2. Adjust the microscope to see the crystals (bottomside) and decide which one you are going to pick-up (keep in mind that you are going to use cryo-loop, so you need enough space between the cover glass and bottom of the microscope) [please look at the attachements for quick understanding] 3. Use the cryoloop from the bottom of the coverglass and pick the crystals. moving the crystals might be easier... all the best. With regards, Kumarevel
-----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@jiscmail.ac.uk] On Behalf Of Wataru Kagawa Sent: Wednesday, February 09, 2011 11:06 AM To: CCP4BB@jiscmail.ac.uk Subject: [ccp4bb] Detaching crystals from glass cover slides Hi all, I have crystals growing by the hanging-drop method, using 24-well VDX plates and Hampton Research siliconized glass cover slides. Most crystals are attached to the cover slide, and I am having difficulties detaching the crystals (using a cryoloop) without breaking them. There are few smaller crystals floating in the drop, and they diffract X-rays pretty well (clean spots, ~3.5A resolution using RAXIS). However, I would like to try the bigger ones, because they may diffract to a higher resolution. Any suggestions for detaching crystals from cover slides will be greatly appreciated. Wataru
cryoloop.ppt
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