Hi,
maybe you solved your problem,
if not, you can try this : You prepare your protein at high salt
concentration. After that you add your ssDNA directly and you proceed by
dialysis. You prepare your dialysis with a low salt concentration
buffer. You can use different buffer by step in order
Dear dengzq1987 (Gmail Research Institute?)
You could use ammonium acetate as the solubilizing salt. It evaporates as
ammonia and acetic acid, so that the ionic strength is gradually reduced by
vapor diffusion. You would then have e.g. PEG in the reservoir and added to the
drop, and you could a
Hi all,
I want to sovle the structrue of ssDNA -protein complex.but the protein are
unstable at low salt concentration,so we use 1M Nacl in the buffer. the high
salt content may disrupt the complex.and I
don't know which ssDNA lenght try fist.any suggestion or experience are
welcome.
thank
