Hi Jay,
Are you sure the missing part of the ligand is disordered and not
cleaved? Depending on how precious the enzyme and the ligand are,
it might be worth denaturing the crystals, centrifugally filtering
the unfolded protein and running mass spec on the flow-through.
My vote is build what
Hi everyone,
I have two types of ligands cocrystallized with my protein, ligand 1 and ligand
2. I am only interested in resolving ligand 2. In my electron density map, only
part of ligand 1 can be identified. Is it ok for me to cut ligand 1 so only the
identifiable part is left or should I keep
