Re: [ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein

2008-01-02 Thread John Berrisford
Juan Sanchez Weatherby wrote: The fact that you cannot find structures with NADH is because from the crystallographic point of view, as you cannot see the hydrogens, then both NAD+ and NADH are the same structure and are thus labeled as "NAD" in a PDB. Therefore if you are looking for a NADH

Re: [ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein

2007-12-31 Thread Juan Sanchez Weatherby
Dear Buz, I may be wrong in this but I think there's no difference between NADH and NAD2H and there are lots of structures on the PDB  databank for both structures with either NAD+ or NADH (upto about 600) and also several with NADP+ or NADPH. A redox reaction involving NAD+ is a 2 electron a

[ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein

2007-12-29 Thread John Tanner
PMID: 16934832 [PubMed - indexed for MEDLINE] > From: Buz Barstow <[EMAIL PROTECTED]> > Reply-To: Buz Barstow <[EMAIL PROTECTED]> > Date: Sat, 29 Dec 2007 10:55:32 -0500 > To: > Subject: [ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein > > Dear A

[ccp4bb] Crystallographic Experiment with NADH and a Flavoprotein

2007-12-29 Thread Buz Barstow
Dear All, I'm planning an experiment to study the oxidation of NADH by a flavoprotein at cryogenic temperatures to facilitate collection of X- ray diffraction data. In planning this experiment, I have seen a few obstacles that I am looking for help in overcoming. 1. There are no structur