Does your system eliminate the need for cryoprotection?
Since the method removes water on the crystal before freezing, in some
cases, we can go with lower concentration of cryoprotectant, like
Paratone-N. But I usually use cryoprotectant for safety.
Also, how do you time the buffer-remova
Patrick Loll
To: CCP4BB@JISCMAIL.AC.UK
Sent: Friday, March 27, 2009 8:35 AM
Subject: [ccp4bb] Fwd: [ccp4bb] Crystal vacuum cleaner
Pretty cool, but the examples shown are all gigantic. Having just
spent a frustrating several hours chasing 5 um crystals, I'd give
half my kingdom for a simple
As an undergrad late in the last century, I used a micromanipulator,
quartz capillaries, and a device similar to a patch pipet (manually
operated via a screw) to do just this. It was just about the time that
nylon loops were coming into wide use, though, and I gladly abandoned he
capillary method
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
***
- Original Message -
From: "Watanabe Nobuhisa"
To:
Cc: "Jacob Keller"
Sent: Thursday, March 26, 2009 10:50 PM
Subject: Re: [ccp4bb] Cr
the dewar?
Jacob
- Original Message -
From: Patrick Loll
To: CCP4BB@JISCMAIL.AC.UK
Sent: Friday, March 27, 2009 8:35 AM
Subject: [ccp4bb] Fwd: [ccp4bb] Crystal vacuum cleaner
Pretty cool, but the examples shown are all gigantic. Having just spent a
frustrating sev
Date: March 27, 2009 8:41:53 AM EDT
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Crystal vacuum cleaner
Reply-To: Patrick Shaw Stewart
Jacob
Have you seen the Crystal Catcher system, developed in Japan?
http://adsabs.harvard.edu/abs/2008APExp...1c7002K
Some of us saw it at a recent IUCr meeting,
letin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Jacob Keller
Sent: 26 March 2009 19:43
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal vacuum cleaner
Dear Crystallographers,
Has anybody ever heard of mounting crystals in tiny crystal-sized
capillaries, such as are pulled by patch-pipet
I am not sure this is the answer for your question, but please check,
http://www.nusrc.nagoya-u.ac.jp/WatanabeLab/XtalMount/index.html
We named it as a "loopless" mounting method in the paper, but now we
leave the loop...
The vacuum cleaner I have used for the method is "my mouth". But we
h
: Thursday, March 26, 2009 2:43 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystal vacuum cleaner
Dear Crystallographers,
Has anybody ever heard of mounting crystals in tiny crystal-sized
capillaries, such as are pulled by patch-pipet machines, or those used in
microfluidics? The material
Problems installing tasks to CCP4i.Dear Crystallographers,
Has anybody ever heard of mounting crystals in tiny crystal-sized capillaries,
such as are pulled by patch-pipet machines, or those used in microfluidics? The
material could be either glass or plastic, and one could have some method of
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