ubject: Re: [ccp4bb] Concentrating protein
Hi there
I quite like the Amicon stirred ultra concentration cell systems. You
can put large volumes in, maximum 1 litre size, I think. As well you
can attach an inert gas such as Argon or Nitrogen, for the gaseous
pressure, this reduces oxidation of y
6372 (office)
>> (904) 953-0046 (lab)
>>
>> -Original Message-
>> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
>> Gina Clayton
>> Sent: Friday, June 27, 2008 7:37 PM
>> To: CCP4BB@JISCMAIL.AC.UK
>> Subject: Re: [ccp4bb] Concen
riday, June 27, 2008 7:37 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Concentrating protein
Hi there
I quite like the Amicon stirred ultra concentration cell systems. You
can put large volumes in, maximum 1 litre size, I think. As well you
can attach an inert gas such as Argon or Nitrogen, fo
Gina Clayton
Sent: Friday, June 27, 2008 7:37 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Concentrating protein
Hi there
I quite like the Amicon stirred ultra concentration cell systems. You
can put large volumes in, maximum 1 litre size, I think. As well you
can attach an inert gas such as
Hi there
I quite like the Amicon stirred ultra concentration cell systems. You
can put large volumes in, maximum 1 litre size, I think. As well you
can attach an inert gas such as Argon or Nitrogen, for the gaseous
pressure, this reduces oxidation of your sample while it
concentrates. M
afterwards. The whole procedure in the end might be
> >> more annoying than just loading a very large volume onto an
> >> ion exchange column.
> >>Phoebe
> >>
> >>
> >> Original message
> >>
> >>> Date:
afterwards. The whole procedure in the end might be
more annoying than just loading a very large volume onto an
ion exchange column.
Phoebe
Original message
Date: Fri, 27 Jun 2008 10:16:53 -0500
From: "First, Eric" <[EMAIL PROTECTED]>
Subject: Re: [ccp4bb]
10:16:53 -0500
> >From: "First, Eric" <[EMAIL PROTECTED]>
> >Subject: Re: [ccp4bb] Concentrating protein
> >To: CCP4BB@JISCMAIL.AC.UK
> >
> > Back in the old days, we used to put the protein in
> > dialysis tubing and surround it with solid NaCl (a 2
&g
batch that is
the way to go as far as time is concerned, as suggested previously.
Jeremy Lohman
University of Oregon
- Original Message -
From: First, Eric
To: CCP4BB@JISCMAIL.AC.UK
Sent: Friday, June 27, 2008 8:16 AM
Subject: Re: [ccp4bb] Concentrating protein
Back in the
end might be
more annoying than just loading a very large volume onto an
ion exchange column.
Phoebe
Original message
>Date: Fri, 27 Jun 2008 10:16:53 -0500
>From: "First, Eric" <[EMAIL PROTECTED]>
>Subject: Re: [ccp4bb] Concentrating protein
>To: C
cular Biology
LSU Health Sciences Center in Shreveport
-Original Message-
From: CCP4 bulletin board on behalf of Exec
Sent: Thu 6/26/2008 10:28 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Concentrating protein
Dear All,
we have GCSF protein produced in inclusion bodies. we solubili
Guenter Fritz wrote:
A mild and quick method is to use dry Sephadex G-25. The material will
swell and take up all the liquid except molecules larger than ca. 5 kDa.
Dear All,
we have GCSF protein produced in inclusion bodies. we solubilise it refold
it and then concentrate it using proflux
A mild and quick method is to use dry Sephadex G-25. The material will
swell and take up all the liquid except molecules larger than ca. 5 kDa.
Dear All,
we have GCSF protein produced in inclusion bodies. we solubilise it refold
it and then concentrate it using proflux system. still the conce
Nathan Cowieson:
I have found that ion exchange is a good way to concentrate protein. If
there is no reason that you can't pass the whole 450 mls through the
column then you should do that.
Another way is to load dilute protein onto small hydroxylapatite column.
Provided that the buffer does
Date: 27 June 2008 1:28:59 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Concentrating protein
Reply-To: Exec <[EMAIL PROTECTED]>
Dear All,
we have GCSF protein produced in inclusion bodies. we solubilise it
refold
it and then concentrate it using proflux system. still the
concentratio
MAIL PROTECTED] On Behalf Of Exec
Sent: Thursday, June 26, 2008 11:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Concentrating protein
Dear All,
we have GCSF protein produced in inclusion bodies. we solubilise it refold
it and then concentrate it using proflux system. still the concentration
Dear All,
we have GCSF protein produced in inclusion bodies. we solubilise it refold
it and then concentrate it using proflux system. still the concentration
of the protein we get is less and volume is more for us to load in Ion
exchange chromatography. is there any simple technique that can be
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