Re: [ccp4bb] Collecting native data at an absorption edge

2012-11-20 Thread Pete Meyer
native datasets? And would a native dataset for refinement be compromised by friedel pair differences collected at an edge? If you've got anomalous data available, you may get better results by using your friedel pair differences in refinement - especially if your diffraction stays in the ~ 4

Re: [ccp4bb] Collecting native data at an absorption edge

2012-11-20 Thread Bosch, Juergen
You'll see the zinc also at the Semet peak edge already check out Ethan's edge plot web server. If you used his-tag purification methods it would be wiser to collect passed the edge of either Ni or Co so you can distinguish them from Zn sites. Jürgen .. Jürgen Bosch Johns

[ccp4bb] Collecting native data at an absorption edge

2012-11-20 Thread Alan Cheung
Dear all - we have a synchrotron trip coming up and have crystals that are likely to contain intrinsically bound zinc atoms. The crystals diffract to 4A-ish so far. We're hoping to collect both native datasets at higher dose, and phasing datasets at lower dose (on separate crystals). Can we