You'll see the zinc also at the Semet peak edge already check out Ethan's edge plot web server. If you used his-tag purification methods it would be wiser to collect passed the edge of either Ni or Co so you can distinguish them from Zn sites.
Jürgen ...................... Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-4742 Lab: +1-410-614-4894 Fax: +1-410-955-3655 http://lupo.jhsph.edu On Nov 20, 2012, at 9:26, "Alan Cheung" <che...@lmb.uni-muenchen.de> wrote: > Dear all - we have a synchrotron trip coming up and have crystals that > are likely to contain intrinsically bound zinc atoms. The crystals > diffract to 4A-ish so far. We're hoping to collect both native datasets > at higher dose, and phasing datasets at lower dose (on separate > crystals). Can we do the lot at the zinc edge wavelength, or should we > be worried about radiation damage, and move away from the edge for the > native datasets? And would a native dataset for refinement be > compromised by friedel pair differences collected at an edge? > > Alan > > > > > -- > Alan Cheung > Gene Center > Ludwig-Maximilians-University > Feodor-Lynen-Str. 25 > 81377 Munich > Germany > Phone: +49-89-2180-76845 > Fax: +49-89-2180-76999 > E-mail: che...@lmb.uni-muenchen.de
