Hi Mark,
Proper co-expression in trans requires that you use plasmids each with
different origins of replication and antibiotic markers, such as p15 +
Kan and ColE1 + Amp. I think the pET vectors of the DUET system use
these two origins (p15 and ColE1). This ensures much more efficient
ut, it can be made to work.
Alas, not in my case!
CC: CCP4BB@JISCMAIL.AC.UK
From: [EMAIL PROTECTED]
Subject: Re: [ccp4bb] Co-expression plasmids
Date: Wed, 2 Apr 2008 19:59:09 +0200
To: [EMAIL PROTECTED]
We have had good experience with the awfully simple minded approach of using
two pET ve
: Transform two plasmids into cells, either
sequentially or
co-transform. I just play down the amount of each DNA to be used.
Raji
-Included Message--
>Date: 2-apr-2008 09:56:09 -0400
>From: "Mark J. van Raaij" <[EMAIL PROTECTED]>
>To:
>Subject: [cc
cp4bb] Co-expression plasmids
To: CCP4BB@JISCMAIL.AC.UK
Dear All,
Anyone have experience with the NovaGen Duet co-expression vectors?
Or can recommend others?
http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
Greetings,
Mark
Mark J. van Raaij
Dpto de Bioquímica, Facultad de Far
d one construct untagged, and the
other with a cleavable His-tag.
Cheers
AGS
Date: Wed, 2 Apr 2008 15:55:27 +0200
From: [EMAIL PROTECTED]
Subject: [ccp4bb] Co-expression plasmids
To: CCP4BB@JISCMAIL.AC.UK
Dear All,Anyone have experience with the NovaGen Duet co-expression vectors? Or
can recommend o
e: 2-apr-2008 09:56:09 -0400
>From: "Mark J. van Raaij" <[EMAIL PROTECTED]>
>To:
>Subject: [ccp4bb] Co-expression plasmids
>
>Dear All,
>
>Anyone have experience with the NovaGen Duet co-expression vectors? Or
>can recommend others?
>http://www.emdb
Dear All,
Anyone have experience with the NovaGen Duet co-expression vectors? Or
can recommend others?
http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
Greetings,
Mark
Mark J. van Raaij
Dpto de Bioquímica, Facultad de Farmacia
Universidad de Santiago
15782 Santiago de Compostela
Spain
