I thought only I was having trouble with cloning into pETDuet1. But just to add to what someone just said.... Yes, I had hell with trying to get one of my ~2kb fragments into pETDuet1 (5.4kb). Could be a combination of vector size and insert size.. Who knows!
Sometimes, I do what Tasos says: Transform two plasmids into cells, either sequentially or co-transform. I just play down the amount of each DNA to be used. Raji ---------Included Message---------- >Date: 2-apr-2008 09:56:09 -0400 >From: "Mark J. van Raaij" <[EMAIL PROTECTED]> >To: <CCP4BB@JISCMAIL.AC.UK> >Subject: [ccp4bb] Co-expression plasmids > >Dear All, > >Anyone have experience with the NovaGen Duet co-expression vectors? Or >can recommend others? >http://www.emdbiosciences.com/html/NVG/Duet_Spot.html > >Greetings, > >Mark > >Mark J. van Raaij >Dpto de Bioquímica, Facultad de Farmacia >Universidad de Santiago >15782 Santiago de Compostela >Spain >http://web.usc.es/~vanraaij/ > > > > > > ---------End of Included Message----------
