Re: [ccp4bb] Changes in Cell Constants

2009-10-23 Thread Ian Tickle
Hi Jacob That doesn't surprise me at all, though the example you heard is probably towards the extreme end of what we've seen. We have seen individual cell parameter changes up to 10% on soaking/freezing which could easily add up to 20-30% change in cell volume. One problem with freezing is that

[ccp4bb] Changes in Cell Constants

2009-10-22 Thread Jacob Keller
Dear Crystallographers, I have just returned from a seminar in which Robert Sauer said that they saw a change in cell volume by 25% upon soaking crystals with substrate (ATP-gammaS). He also showed that one of the cell constants changed by about -25 Ang from 200 Ang. I am assuming that the spa