@JISCMAIL.AC.UK] On Behalf Of Jrh
Sent: 31 October 2012 08:20
To: ccp4bb
Subject: Re: [ccp4bb] Ca or Zn
Dear Ethan,
Yes indeed.
An exciting development underway at Diamond Light Source led by Armin Wagner
will greatly improve the ease of measurement at eg the calcium edge but also
extending that
wrote:
>>>>
>>>>> Veerendra,
>>>>>
>>>>> You can rule out if zinc has replaced calcium ions (although I agree with
>>>>> Nat and others that looking at the coordination sphere should give a big
>>>>> clue) by
ue.
> >>> You can learn how many zinc, if any, have bound per one protein molecule
> >>> in the dissolved crystal.
> >>>
> >>> Best
> >>> Chitta
> >>>
> >>>
> >>>
> >>> - Original
ve bound per one protein molecule in
>>> the dissolved crystal.
>>>
>>> Best
>>> Chitta
>>>
>>>
>>>
>>> - Original Message -
>>> From: "Veerendra Kumar"
>>> To: CCP4BB@JISCMAIL.
nd per one protein molecule in
> > the dissolved crystal.
> >
> > Best
> > Chitta
> >
> >
> >
> > - Original Message -----
> > From: "Veerendra Kumar"
> > To: CCP4BB@JISCMAIL.AC.UK
> > Sent: Tuesday, October 30
iginal Message -
> From: "Veerendra Kumar"
> To: CCP4BB@JISCMAIL.AC.UK
> Sent: Tuesday, October 30, 2012 2:55:33 PM
> Subject: [ccp4bb] Ca or Zn
>
> Dear CCP4bb users,
>
> I am working on a Ca2+ binding protein. it has 4-5 ca2+ binding sites. I
> purified t
technique. You can learn how many
zinc, if any, have bound per one protein molecule in the dissolved crystal.
Best
Chitta
- Original Message -
From: "Veerendra Kumar"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, October 30, 2012 2:55:33 PM
Subject: [ccp4bb] Ca or Zn
Dear CCP4bb us
Do occupancy refinement. Especially if you use the new "anomalous
refinement" option available in refmac, phenix.refine and other packages
as well, you should be able to get a pretty reliable number for how many
"real electrons" as well as "anomalous electrons" are at each site. At
almost any
same
> locations?
>
>
> From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Kumar,
> Veerendra [veerendra.ku...@uconn.edu]
> Sent: Tuesday, October 30, 2012 1:55 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Ca or Zn
>
&g
On 10/30/12 15:02, Bosch, Juergen wrote:
calculate an anomalous map, you should see the Zn signal even if you
collected at the SeMet peak.
Jürgen
..
Ca can have a noticeable anomalous signal of its own, if your data are good.
If the possibility to collect new data exists,
board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Kumar, Veerendra
> Sent: Tuesday, October 30, 2012 19:56
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Ca or Zn
>
> Dear CCP4bb users,
>
> I am working on a Ca2+ binding protein. it has 4-5 ca2+ binding sites. I
> pur
On Tue, Oct 30, 2012 at 12:12 PM, Jim Pflugrath
wrote:
> How would you distinguish between a mixture of Ca and Zn in the same
> locations?
How often would they be likely to bind in the same place? Some of the
other transition metals are difficult to tell apart, but Ca and Zn
have very different
Subject: [ccp4bb] Ca or Zn
Dear CCP4bb users,
I am working on a Ca2+ binding protein. it has 4-5 ca2+ binding sites. I
purified the protein in presence of Ca2+ and crystallized the Ca2+ bound
protein. I got crystal and solved the structure by SAD phasing at 2.1A
resolution. I can see the clear
calculate an anomalous map, you should see the Zn signal even if you collected
at the SeMet peak.
Jürgen
..
Jürgen Bosch
Johns Hopkins University
Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria Research Institute
615 North
Dear CCP4bb users,
I am working on a Ca2+ binding protein. it has 4-5 ca2+ binding sites. I
purified the protein in presence of Ca2+ and crystallized the Ca2+ bound
protein. I got crystal and solved the structure by SAD phasing at 2.1A
resolution. I can see the clear density in the difference
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