Re: [ccp4bb] Amazing B-factor

2009-06-01 Thread Dale Tronrud
Dear Jain, The Wilson plot is based on the assumption that you can model your structure with a collection of atoms randomly distributed in the unit cell. This model is very poor at low resolution, say, 4.5A and lower. Since the Wilson B depends on the change in scattering as a function of reso

[ccp4bb] Amazing B-factor

2009-06-01 Thread Jian Wu
Dear all, Recently we have collected one set of data which is processed to 2.9A and 3.0A and the Wilson-B values are 50.1 and 59.1, respectively. As for the completeness of the highest shell is only 66% in 2.9A (80% in 3.0A), we use the dataset with 3.0A for phasing and refinement. Everything is

[ccp4bb] Amazing B-factor

2009-06-01 Thread Jian Wu
Dear all, Recently we have collected one set of data which is processed to 2.9A and 3.0A and the Wilson-B values are 50.1 and 59.1, respectively. As for the completeness of the highest shell is only 66% in 2.9A (80% in 3.0A), we use the dataset with 3.0A for phasing and refinement. Everything is O