Dear all, Recently we have collected one set of data which is processed to 2.9A and 3.0A and the Wilson-B values are 50.1 and 59.1, respectively. As for the completeness of the highest shell is only 66% in 2.9A (80% in 3.0A), we use the dataset with 3.0A for phasing and refinement. Everything is OK during phasing. Routinely, we use CNS (rigid, minimize and bgroup) for primary refinement. -- Jian Wu
Ph.D. Student Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences (CAS) Tel: 0086-21-54921117 Email: prote...@gmail.com
