Message-
From: Michael C. Wiener
Sent: Friday, February 21, 2014 12:16 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Aggregation assay
Analogous to Dr. Mirella, we've used the ratio of A320/A280 (for membrane
proteins). In response to a fussy reviewer, I located some relevant
re
Analogous to Dr. Mirella, we've used the ratio of A320/A280 (for membrane
proteins). In response to a fussy reviewer, I located some relevant references
(refs. 10-13, copied below) when we referred to this in "A high-throughput
differential filtration assay to screen and select detergents
for me
I am aware of an assay for aggregation (aggregation rate) that is based on
fluorescence measurements. It involves excitation at 280 and emission in the
range 260-400. Is there a reference for the absorbance method?
See
Nominé Y, Ristriani T, Laurent C, Lefèvre JF, Weiss E, Travé G. A strategy
