Re: [ccp4bb] Adding ligand to crystallization drop

2013-10-03 Thread Saugata Hazra
USA             From: Christopher Browning To: CCP4BB@JISCMAIL.AC.UK Sent: Thursday, September 12, 2013 6:25 AM Subject: Re: [ccp4bb] Adding ligand to crystallization drop Hi Again, Thanks for the suggestions. I just tried it at 2 ligand concentrations and the crystals

Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-13 Thread Roessler, Christian
4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Christopher Browning Sent: Thursday, September 12, 2013 10:34 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Adding ligand to crystallization drop Thanks for the really helpful tips. Right now I've tried 5, 10 and 16% DMSO (due t

Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-12 Thread Bosch, Juergen
But you have not tested in the beam if the visually happy crystals diffract right ? I would do this first before consuming your precious ligands in worthless drops. Jürgen On Sep 12, 2013, at 10:34 AM, Christopher Browning wrote: Right now I've tried 5, 10 and 16% DMSO (due to variations in the

Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-12 Thread Christopher Browning
jhsph.edu>> Date: Thursday, 12 September 2013 15:09 To: Christopher Browning mailto:christopher_b_brown...@vrtx.com>> Cc: "CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>" mailto:CCP4BB@JISCMAIL.AC.UK>> Subject: Re: [ccp4bb] Adding ligand to crystalli

Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-12 Thread Bosch, Juergen
_b_brown...@vrtx.com] Sent: Thursday, September 12, 2013 4:37 AM To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Adding ligand to crystallization drop Hi, I've just got a quick question about getting ligands bound to proteins in crystals. I've managed to co-crys

Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-12 Thread Christopher Browning
o: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK> Subject: [ccp4bb] Adding ligand to crystallization drop Hi, I've just got a quick question about getting ligands bound to proteins in crystals. I've managed to co-crystallize my proteins with the various ligands, and I'

Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-12 Thread Noinaj, Nicholas (NIH/NIDDK) [E]
lto:noin...@niddk.nih.gov> [ the Buchanan Lab ] http://www-mslmb.niddk.nih.gov/buchanan/ From: Christopher Browning [mailto:christopher_b_brown...@vrtx.com] Sent: Thursday, September 12, 2013 4:37 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Adding ligand to crystallization drop Hi, I&

[ccp4bb] Adding ligand to crystallization drop

2013-09-12 Thread Christopher Browning
Hi, I've just got a quick question about getting ligands bound to proteins in crystals. I've managed to co-crystallize my proteins with the various ligands, and I'm aware that soaking will work as well but I want to speed up the process. Would this work? If I had a bunch of crystals that have g