se Antonio Cuesta-Seijo
Sent: Monday, December 08, 2008 12:54 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AW: [ccp4bb] suggestions for UV spectrometer
I also have to come in defense of the nanodrop here.
I have measured up to A280 = 98 and the curve is always reasonably
smooth, spikes normally
I also have to come in defense of the nanodrop here.
I have measured up to A280 = 98 and the curve is always reasonably
smooth, spikes normally mean that bubbles have formed. And "proper
cleaning" seems to be rubbing with a kimwipe three or four times
after each drop. If the last user does n
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Juergen Bosch
Sent: Saturday, December 06, 2008 12:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AW: [ccp4bb] suggestions for UV spectrometer
Hi,
really strange, I always dilute my protein when taking an absorption
Hi,
really strange, I always dilute my protein when taking an absorption
spectra. I try to adjust my expected concentration to a readout of ~
0.2-0.3 OD280. And the Bradford is just as well as 'picking house
numbers', depending on your protein, you can underestimate your
protein concentra
Dear all,
Thanks a lot for raising the issue with the not reproducibility of protein
measurement with Nanodrop. We use the instrument as a workhorse in the lab.
Indeed, recently I observed that the protein concentration suggested by
Nanodrop is sometimes differ to the usual colorimetric measuremen
Which brings us back to the Hellma "TrayCell" solution where you can,
from the same spectrometer, have both the cuvette option and the
quickness of the NanoDrop/NanoVue system.
Anyone that can comment on the performance of the TrayCell from Hellma?
Cheers,
Martin
On Dec 5, 2008, at 9:06 AM
Agree!
I think for crystallographic use the nanodrop is perfectly okay to see if
the protein is 5mg/ml or 30mg/ml. But in fact I also do not trust our
instrument if it comes to more important issues like preparing solutions for
titrations or assays. And due to the small pathlength I do not trust
